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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | Recombinant Carboxypeptidase B |
| Minimum Order Quantity: | 1mg |
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| Price: | $67/mg |
| Packaging Details: | 1mg, 10mg, 100mg; ice packing, carton |
| Delivery Time: | 2-3 work days |
| Payment Terms: | T/T |
| SOURCE: | Recombinant E. Coli | Appearance: | White Or Off White, Or Yellowish Powder |
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| Specific Activity: | ≥170 Units/mg Pro. | Purity(SDS-PAGE): | Single Major Band |
| Molecular Weight(SDS-PAGE): | 33.8±3.4 KDa | Cas: | 9025-24-5 |
| Source: | Recombinant Carboxypeptidase B Is A Genetically Engineered Protein Expressed In E.coli | ||
| High Light: | sequencing grade modified trypsin,carboxypeptidase enzyme |
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Recombinant Carboxypeptidase B, Catalyzes Hydrolysis of Basic Amino Acids Lysine, Arginine and Histidin
Recombinant Carboxypeptidase B
Recombinant Carboxypeptidase B
Catalog Number:RCPB01
CAS: 9025-24-5
EC: 3.4.17.2
Storage temperature: 2-8℃
Source: Recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B
1.DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.
2.MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | White or off white, or yellowish powder |
| Specific activity | ≥170 units/mg pro. |
| Purity(SDS-PAGE) | Single major band |
| Molecular Weight(SDS-PAGE) | 33.8±3.4 kDa |
UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
(1)Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.
(2)Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
(3)High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host protein residues is less than the limits of biological products.
(4)Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
(5)Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
The production of recombinant insulin and its analogues; the quality test of recombinant antibodies; the determination of C-terminal amino acids of proteins; the production of other recombinant polypeptides; and the enzymatic synthesis of certain specific compounds.
7.RELATED PRODUCTS
Recombinant trypsin
Sequencing grade trypsin
Recombinant human trypsin
Recombinant enterokinase
Contact Person: Miss Eland
Tel: +8613482039151
