High Purity, Recombinant Kex2 Protease, Purified by HPLC

High Purity, Recombinant Kex2 Protease, Purified by HPLC

Recombinant Kex2 Protease

Synonyms: Endoproteinase Lys/Arg-Arg; Kex2 Protease;YSCF Protenase

Source: Recombinant Kex2 Protease is a genetically engineered protein expressed in Pichia pastoris

Storage temperature: 2-8℃

Protein Sequence:The amino acid sequence of recombinant Kex2 Protease is identical to Saccharomyces cerevisiae Kex2

Kex2 is a Ca²⁺-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃.It is stable in buffer (pH 5.0-6.0).The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.

Unit Definition:One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA as the substrate.