Place of Origin: | China, Shanghai |
Brand Name: | YAXINBIO |
Certification: | NQA ISO 9001:2015 |
Model Number: | Re15 |
Minimum Order Quantity: | 10U |
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Price: | 545USD/mg |
Packaging Details: | ice packaging, carton |
Payment Terms: | T/T |
Brand: | YaxinBio | Source: | Pichia Pastoris |
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Appearance: | White Or Off White Powder | Specific Activity: | ≥10 Units/mg Pro. |
Purity(SDS-PAGE): | Single Major Band | Molecular Weight(SDS-PAGE): | 67 ± 6.7 KDa |
High Light: | Recombinant protease,saccharomyces cerevisiae yeast |
High Purity, Recombinant Kex2 Protease, Purified by HPLC
Recombinant Kex2 Protease
Synonyms: Endoproteinase Lys/Arg-Arg; Kex2 Protease;YSCF Protenase
Source: Recombinant Kex2 Protease is a genetically engineered protein expressed in Pichia pastoris
Storage temperature: 2-8℃
Protein Sequence:The amino acid sequence of recombinant Kex2 Protease is identical to Saccharomyces cerevisiae Kex2
Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃.It is stable in buffer (pH 5.0-6.0).The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.
Purified by | HPLC |
Format | Liquid in10mM NaAc-HAc(PH 5.2) and 2mM Ca2+ |
Specific activity | ≥10u/mg |
12.5%SDS-PAGE | Single main stripe |
Mol.weight | 67±6.7 kDa |
Unit Definition:One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA as the substrate.
Contact Person: Miss Eland
Tel: +8613482039151