Kex-2 Protease Double Base Enzyme that the Optimum PH is 9.0 With 2-8°C Storage Temperature

Kex-2 Protease Double Base Enzyme that the Optimum PH is 9.0 With 2-8°C Storage Temperature

Kex-2 Protease

PRODUCT INFORMATION
Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids.At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.

MAIN FEATURES

UNIT DEFINITION: One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA (Boc-Gln-Arg-Arg-pNA) as the substrate.

RECOMMEND USAGE

Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl,2 mM Ca^{2 +} or HEPES, 5 mM Ca^{2 +}. If it is not used immediately after dissolving, it is recommended to dissolve the powder with 20 mM (pH 5.2) NaAc-HAc and 2 mM Ca^{2 +} buffer to make the final concentration is about 1-10 mg/ml,then make suitable packing as your requirement and store at -20℃.Reaction was carried out, using pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca^{2 +} or HEPES, 5 mM Ca^{2 +} buffer.

Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0.