Place of Origin: | China, Shanghai |
Brand Name: | YAXINBIO |
Certification: | NQA ISO 9001:2015 |
Model Number: | Re15 |
Minimum Order Quantity: | 50ug |
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Price: | USD142 |
Packaging Details: | 50ug |
Delivery Time: | 4days |
Payment Terms: | T/T |
Supply Ability: | 10000G |
Activity: | ≥10u/mg | Purification: | HPLC |
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Character: | Freeze-dried Powder | Molecular: | 67±6.7 KDa |
Perfect PH: | 9.0 | Nature: | Double Base Enzyme |
Temperature: | 2-8℃ | ||
High Light: | chemical ingredients,saccharomyces cerevisiae yeast |
Kex-2 Protease Double Base Enzyme that the Optimum PH is 9.0 With 2-8°C Storage Temperature
Kex-2 Protease
PRODUCT INFORMATION
Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids.At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.
MAIN FEATURES
Source | Pichia pastoris |
Appearance | White or off white powder |
Specific activity | ≥10.0 units/mg pro. |
Purity(SDS-PAGE) | Single major band |
Molecular Weight(SDS-PAGE) | 67±6.7 kDa |
UNIT DEFINITION: One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA (Boc-Gln-Arg-Arg-pNA) as the substrate.
RECOMMEND USAGE
Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl,2 mM Ca2 + or HEPES, 5 mM Ca2 +. If it is not used immediately after dissolving, it is recommended to dissolve the powder with 20 mM (pH 5.2) NaAc-HAc and 2 mM Ca2 + buffer to make the final concentration is about 1-10 mg/ml,then make suitable packing as your requirement and store at -20℃.Reaction was carried out, using pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2 + or HEPES, 5 mM Ca2 + buffer.
Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0.
Contact Person: Miss Eland
Tel: +8613482039151