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Recombinant Carboxypeptidase B, Proteomics Grade, Enzyme purity 200U/mg Pro.

Recombinant Carboxypeptidase B, Proteomics Grade, Enzyme purity 200U/mg Pro.

  • Recombinant Carboxypeptidase B, Proteomics Grade, Enzyme purity 200U/mg Pro.
  • Recombinant Carboxypeptidase B, Proteomics Grade, Enzyme purity 200U/mg Pro.
  • Recombinant Carboxypeptidase B, Proteomics Grade, Enzyme purity 200U/mg Pro.
  • Recombinant Carboxypeptidase B, Proteomics Grade, Enzyme purity 200U/mg Pro.
Recombinant Carboxypeptidase B, Proteomics Grade, Enzyme purity 200U/mg Pro.
Product Details:
Place of Origin: China, Shanghai
Brand Name: YAXINBIO
Certification: NQA ISO 9001:2015
Model Number: SRCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 1mg
Price: 385$/mg
Packaging Details: 1mg, 10mg, 100mg, 1g; dry ice, carton
Delivery Time: five days
Payment Terms: T/T
Supply Ability: 1000g per week
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Detailed Product Description
Product: Sequencing Grade Carboxypeptidase B EC: 3.4.17.2
CAS: 9025-24-5 Specific Activity: NLT 200 Units/mg Pro
Contaminant Activity: No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Recombinant Trypsin (sequencing Grade)
High Light:

trypsin endopeptidase

,

carboxypeptidase enzyme

Recombinant Carboxypeptidase B, Proteomics Grade, Enzyme purity 200U/mg Pro.

 

Recombinant Carboxypeptidase B

 

 

Description

 

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The

enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

 

Application

 

Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine

and histidine from the C-terminal end of polypeptides.

Antibody quality control.

 

Main Features

 

Purity/Purification Greater than 90% as determined by SDS-PAGE.
Form/Format The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.
Sterile Filtered lyophilized powder.
Unit Defenition One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25 degree C, pH-7.65.
Solubility It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity 200 units/mg protein.
Preparation and Storage Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Other Notes for Cpb1 recombinant protein Small volumes of Cpb1 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Recommend Usage

 

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratioto aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

 

Storage Instructions

 

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.

 

Recombinant Carboxypeptidase B, Proteomics Grade, Enzyme purity 200U/mg Pro. 0

 

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Mr. zhao

Tel: +86-21-54336492

Fax: 86-21-54336593

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