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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | SRT0201 |
| Minimum Order Quantity: | 100ug |
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| Price: | 98$/100ug |
| Packaging Details: | ice packaging, carton |
| Delivery Time: | 7 weekdays |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Sequencing Grade Trypsin | Cat. No.: | SRT0201 |
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| CAS: | 9002-07-7 | Specific Activity: | >4500 USP Units/mg Pro |
| EC: | 3.4.21.4 | Contaminant Activity: | No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant. |
| High Light: | sequencing grade modified trypsin,trypsin endopeptidase |
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ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys-C for Digesting Specific Protein
Sequencing Grade Trypsin
DESCRIPTION
YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | White or off white, or yellowish powder |
| Specific activity | ≥4500 USP units/mg pro. |
| SDS-PAGE | Single major band |
| Molecular Weight(SDS-PAGE) | 24.0±2.4 kDa |
| Purity(HPLC) | ≥95% |
Unit Definition
One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Application
Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
Reconmend Usage
1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.
2. When using this product, thaw it at room temperature, and mix gently before use.
3. No activity lost when freeze-thaw 5 cycles.
Storage Instructions
1. The solution should be stored under -70℃, It is stable within 24 months.
2. Above 95% activity remained after 5 times repeated freezing and thawing.
3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.
PRODUCT USAGE
The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.
Contact Person: Miss Eland
Tel: +8613482039151
