Place of Origin: | China, Shanghai |
Brand Name: | YAXINBIO |
Certification: | NQA ISO 9001:2015 |
Model Number: | SRCPB01 |
Minimum Order Quantity: | 100ug |
---|---|
Price: | 198$/100ug |
Packaging Details: | 100ug, 1mg, 5mg; ice packaging, carton |
Payment Terms: | T/T |
Supply Ability: | 1000g per week |
Source: | E. Coli | Purified By: | HPLC |
---|---|---|---|
Physical Form: | Lyophilized Powder | Specific Activity: | NLT 200 Units/mg Pro |
Contaminant Activity: | No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. | Cas: | 9025-24-5 |
EC: | 3.4.17.2 | ||
High Light: | sequencing grade modified trypsin,trypsin endopeptidase |
Recombinant Carboxypeptidase B, Sequencing Grade for Mass Spectrometry Analysising lysine Peak Antibody
Recombinant Carboxypeptidase B
Description
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
Recommend Usage
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Storage Instructions
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.
Contact Person: Miss Eland
Tel: +8613482039151