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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RPT0201 |
| Minimum Order Quantity: | 1g |
|---|---|
| Price: | 450$/g |
| Packaging Details: | ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Purified By: | HPLC | Format: | White Lyophilized Powder |
|---|---|---|---|
| Specific Activity: | ≥3800 USP U/mg Pro | Purity(RP-HPLC): | NLT 70% β-trysin, NMT 20% α-trypsin |
| Source: | E. Coli | ||
| High Light: | porcine trypsin,trypsin enzyme |
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Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
Recombinant Trypsin
Description
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified
by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
Calculation
| BAEE Units/ml enzyme = | (ΔA253nm/min Test - ΔA253nm/min Blank)(df) |
| (0.001) (0.20) |
df = Dilution factor
0.001 = The change in A253nm/minute per unit of Trypsin at pH 7.6 at 25 °C in a 3.2 ml reaction
mix 0.20 = Volume (in milliliters) of enzyme used
| Units/mg solid= | units/ml enzyme |
| mg solid/ml enzyme |
Notes
1.This assay procedure is not to be used to assay immobilized trypsins
2.For the USP/NF procedure refer to the USP monograph
3.This procedure is for informational purposes. For a current copy of Sigma’s quality control procedure contact our Technical Service Department.
Unit Conversions
1 TAME µM Unit = 0.27 BAEE µM Units
1 BAEE µM Unit = 3.64 TAME Units
1 TAME µM Unit = 55 BAEE A253 Units
1 BAEE A253 Unit = 0.018 TAME µM Unit
1 TAME µM Unit = 180 TAME A247 Units
1 TAME A247 Unit = 0.33 BAEE Units
1 USP Unit = 3.0 BAEE Units
1 NF Unit = 1.1 USP Units
Advantages
Animal origin free: The use of recombinant Porcine Trypsin eliminates the risk of virus presence,
and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant
Porcine Trypsin belongs to the AOF level 3.
Stablility: A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Recommend Usage
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.
Storage Instructions
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable
within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and
above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland
Tel: +8613482039151
