Trypsin Solution 1 / 2500 Animal Composition Free Trypsin for Cell Dissociation

Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation 

Recombinant trypsin solution(RTS)

Description

Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin  expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.

Method Of Use

Step 1. Pour 1 ml buffer solution into EP tube containing trypsin powder.

Step 2. Dissolve the trypsin powder.

Step 3. Pour the solution into the vial containing buffer solution and mix. Best use it within 4 hours.

Notes

Note 1. Dissociate adherent cells under room temperature ; 37 ℃ is not recommended.

Note 2. If necessary, after dissolving, immediately dispense into suitable ; containers, then stored under -20℃. To use it, dissolve it at room temperature. 

Note 3. The content of trypsin is 2000 BAEE unit/ml as above prepared. If necessary, dilute it to 1000 unit/ml or others based on your experimental results.  

Instructions for use

The following instructions are applicable for most cell lines. Actual procedures and concentrations should be determined by experience with individual cell lines (see notes below).

1. Wash the cells with DPBS w/o Ca, Mg (Cat No. 02-023-1).

2. Add 1ml of Recombinant Trypsin Solution to each T-25 tissue culture flask.

3. Incubate the flask at 37°C for 2 min or longer as necessary. Verify cell’s detachment using inverted microscope.

4. Pellet the cells by centrifugation, and discard the supernatant. 6. Resuspend the cells in growth medium, and seed as desired.