Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

 Recombinant protein A                   

Application

The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.

PRODUCT ADVANTAGE

• Non-antibody column purification, the product without any other antibodies such as IgG and other pollution.
• SPA-25 is our own research and development products, pH tolerance range, can withstand 0.5 M NaOH and 0.5M HCl treatment, no degradation, antibody binding capacity unchanged.
• The product has a specially designed link for the purification of the column, can be directly used for the preparation of protein A column.
• Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
• High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
• Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
• Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
• Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

Storage Instructions

Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.

Usage

For Research or Manufacturing Purpose Only. Not for Human.