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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RSPA05 |
| Minimum Order Quantity: | 10mg |
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| Price: | 79$/10mg |
| Packaging Details: | 10mg, 100mg, 1g; ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Brand: | Yaxinbio | Substrate: | Porous Polystyrene-divinylbenzene |
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| Ligand: | Recombinant Protein A | Recommend Flow Rate: | 300-700 Cm/h |
| DBC: | At Least 300 Mg IgG/mL Media At 500 Cm/h | Maximum Pressure: | 1500 PSI |
| High Light: | SPA protein A,purified protein |
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Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A
Recombinant Protein A
CAS:91932-65-9
Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli,.
Application(1)
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.
APPLICATION(2)
The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.
MAIN FEATURES
| Appearance | White or off white powder |
| Solubility | Clear liquid |
| Bioburden | ≤ 1 cfu/ mg |
| SDS-PAGE | Single major band |
| SDS-PAGE (MW) | 25000±1000 Daltons |
| UV Spectrum | E275/250 ≥ 1.2 |
| E0.1% at 275nm = 0.17-0.23 | |
| Purity (HPLC) | ≥ 95% |
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.
After dissolved with pure water, it should be stored under -20℃.
Stability of transport: The product is stable by blue ice insulation transport.
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Contact Person: Miss Eland
Tel: +8613482039151
