Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry

Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry

Recombinant Carboxypeptidase B

Description

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

Main Features

Purity: Greater than 95% as determined by SDS-PAGE.

Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.

Bilogical Activity:170 units/mg protein.

Application

Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.

Antibody quality control.

Recommend Usage

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

Storage Instructions

Stability:Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below under 2-8℃ in sealed container. For long term storage it is recommended to be stored under -20℃. Please prevent freeze-thaw cycles.