Place of Origin: | China, Shanghai |
Brand Name: | YAXINBIO |
Certification: | NQA ISO 9001:2015 |
Model Number: | RCPB01 |
Minimum Order Quantity: | 100ug |
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Price: | 198$/100ug |
Packaging Details: | ice packaging, carton |
Payment Terms: | T/T |
Supply Ability: | 1000g per week |
Product: | Recombinant Carboxypeptidase B | Manufacturer: | YaxinBio |
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Source: | E. Coli | Purified By: | HPLC |
Contaminant Activity: | No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. | RELATED PRODUCT: | Recombinant Chymotrypsin (sequencing Grade); |
High Light: | trypsin endopeptidase,carboxypeptidase enzyme |
Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry
Recombinant Carboxypeptidase B
Description
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
Main Features
Purity: Greater than 95% as determined by SDS-PAGE.
Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.
Bilogical Activity:170 units/mg protein.
Application
Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.
Antibody quality control.
Recommend Usage
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Storage Instructions
Stability:Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below under 2-8℃ in sealed container. For long term storage it is recommended to be stored under -20℃. Please prevent freeze-thaw cycles.
Contact Person: Miss Eland
Tel: +8613482039151