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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | SRCPB01 |
| Minimum Order Quantity: | 100ug |
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| Price: | 198$/100ug |
| Packaging Details: | 100ug, 1mg, 5mg; ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Sequencing Grade Carboxypeptidase B | Manufacturer: | YaxinBio |
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| Physical Form: | Lyophilized Powder | Specific Activity: | NLT 200 Units/mg Pro |
| Contaminant Activity: | No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. | Molecular Weight(SDS-PAGE): | 33.8±3.4 KDa |
| Purity(HPLC): | ≥ 95% | ||
| High Light: | trypsin endopeptidase,carboxypeptidase enzyme |
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Carboxypeptidase B, Sequencing Grade Carboxypeptidase B, For Mass Spectrometry
1.DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
2. MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | White or off white, or yellowish powder |
| Specific activity | ≥200 units/mg pro. |
| SDS-PAGE | Single major band |
| Molecular Weight(SDS-PAGE) | 33.8±3.4 kDa |
| Purity(HPLC) | ≥ 95% |
Formulation:The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.
Solubility:It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile
18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Purity: Greater than 95% as determined by SDS-PAGE.
Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may
not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Stability
Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below 2-8°C. For long term storage it is recommended to be stored below -20℃.Please prevent freeze-thaw cycles.
Contact Person: Miss Eland
Tel: +8613482039151
