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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | SRT0201 |
| Minimum Order Quantity: | 100ug |
|---|---|
| Price: | 198$/100ug |
| Packaging Details: | ice packaging |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Sequencing Grade Trypsin | Cat. No.: | SRT0201 |
|---|---|---|---|
| Physical Form: | Lyophilized Powder | Specific Activity: | >4500 USP Units/mg Pro |
| Purity: | One Single Main Band At 24kD By SDS-PAGE | Contaminant Activity: | No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant. |
| High Light: | trypsin endopeptidase,serine protease |
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Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis
Sequencing Grade Trypsin
Catalog Number:SRT0201
CAS:9002-07-7
EC:3.4.21.4
Storage temperature: 2-8℃
Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli
Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin
Description
Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
Main Features
|
Source |
E. Coli |
|
Format |
Liquid in 50mM HAc, or fluffy solid. |
|
Protein concentration |
0.5mg/ml in 50mM HAc |
|
Purity(RP-HPLC) |
≥99% by HPLC |
|
Contaminant activity |
Recombinant porcine trypsin. No any other proteases activities contaminant. |
Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
RECOMMEND USAGE
It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.
Contact Person: Miss Eland
Tel: +8613482039151
