 |
| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | SRT0201 |
| Minimum Order Quantity: | 100ug |
|---|---|
| Price: | 198$/100ug |
| Packaging Details: | ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Sequencing Grade Trypsin | Contaminant Activity: | No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant. |
|---|---|---|---|
| Physical Form: | Lyophilized Powder | Specific Activity: | >4500 USP Units/mg Pro |
| Purity: | One Single Main Band At 24kD By SDS-PAGE | RELATED PRODUCT: | Recombinant Protein A |
| High Light: | sequencing grade modified trypsin,serine protease |
||
Sequencing Grade Trypsin, Digestion of Protein and Polypeptide
Sequencing Grade Trypsin (modified)
Description
Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | White or off white, or yellowish powder |
| Specific activity | ≥4500 USP units/mg pro. |
| SDS-PAGE | Single major band |
| Molecular Weight(SDS-PAGE) | 24.0±2.4 kDa |
| Purity(HPLC) |
≥95% |
PRODUCT ADVANTAGE
Sequence grade recombinant protein: high specificity,good stability.
Animal origin free: sequencing grade modified trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
|
Source |
E. Coli |
|
Format |
Liquid in 50mM HAc, or fluffy solid. |
|
Protein concentration |
0.5mg/ml in 50mM HAc |
|
Purity(RP-HPLC) |
≥99% by HPLC |
|
Contaminant activity |
Recombinant porcine trypsin. No any other proteases activities contaminant. |
Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Application
Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
Recommend Usage
1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.
2. When using this product, thaw it at room temperature, and mix gently before use.
3. No activity lost when freeze-thaw 5 cycles.
Storage Instructions
1. The solution should be stored under -70℃, It is stable within 24 months.
2. Above 95% activity remained after 5 times repeated freezing and thawing.
3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.
Contact Person: Miss Eland
Tel: +8613482039151
