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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | SRT0201 |
| Minimum Order Quantity: | 100ug |
|---|---|
| Price: | 198$/100ug |
| Packaging Details: | ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Sequencing Grade Trypsin | Cat. No.: | 9002-07-7 |
|---|---|---|---|
| Packaging: | 100ug 1mg | Manufacturer: | YaxinBio |
| Specific Activity: | ≥4500 USP /mg Pro | RELATED PRODUCT: | Recombinant Carboxypeptidase B |
| High Light: | trypsin endopeptidase,serine protease |
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Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio
Sequencing Grade Trypsin
Description
Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere
with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin
is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
RECOMMEND USAGE
It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.
It is no activity loss after 5 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
Main Features
|
Source |
E. Coli |
|
Format |
Liquid in 50mM HAc, or fluffy solid. |
|
Protein concentration |
0.5mg/ml in 50mM HAc |
|
Purity(RP-HPLC) |
≥99% by HPLC |
|
Contaminant activity |
Recombinant porcine trypsin. No any other proteases activities contaminant. |
Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a
reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Contact Person: Miss Eland
Tel: +8613482039151
