Place of Origin: | China, Shanghai |
Brand Name: | YAXINBIO |
Certification: | NQA ISO 9001:2015 |
Model Number: | SRT0201 |
Minimum Order Quantity: | 100ug |
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Price: | 198$/100ug |
Packaging Details: | ice packaging, carton |
Payment Terms: | T/T |
Supply Ability: | 1000gper week |
Product: | Sequencing Grade Trypsin | Cat. No.: | SRT0201 |
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Packaging: | 100ug 1mg | Manufacturer: | YaxinBio |
Purity: | One Single Main Band At 24kD By SDS-PAGE | Contaminant Activity: | No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant. |
High Light: | sequencing grade modified trypsin,serine protease |
Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
Sequencing Grade Trypsin
Description
Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
Main Features
Source |
E. Coli |
Format |
Liquid in 50mM HAc, or fluffy solid. |
Protein concentration |
0.5mg/ml in 50mM HAc |
Specific activity |
≥ 4500 USP units/mg pro |
Purity(RP-HPLC) |
≥99% by HPLC |
Contaminant activity |
Recombinant porcine trypsin. No any other proteases activities contaminant. |
Unit Definition :One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Application
Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
Recommend Usage
1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.
2. When using this product, thaw it at room temperature, and mix gently before use.
3. No activity lost when freeze-thaw 5 cycles.
1. The solution should be stored under -70℃, It is stable within 24 months.
2. Above 95% activity remained after 5 times repeated freezing and thawing.
3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.
Contact Person: Miss Eland
Tel: +8613482039151