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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RTI16-S |
| Minimum Order Quantity: | 10mg |
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| Price: | 180$/10mg |
| Packaging Details: | 1mg, 10mg, 100mg, 1g; ice packaging, carton |
| Delivery Time: | 5-8 work days |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Recombinant Aprotinin | Cat. No.: | RTI16-S |
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| Source: | E. Coli | Purified By: | HPLC |
| Related Product: | Recombinant Porcine Trypsin | Specific Activity: | ≥ 3EPU /mg Pro |
| High Light: | trypsin inhibitor,aprotinin protease inhibitor |
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Inhibit Plasmin, 3 EPU/mg pro., Aprotinin from Bovine Lung, Inhibit Kallikrein
Recombinant Aprotinin
Description
Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes and dissociating at pH >10 or <3. Effective concentration is equimolar with protease.
Recombinant Animal Components Free
Recombinant aprotinin is expressed in E. Coli, and purified by HPLC. It contains no animal-derived components. This is a recombinant form of bovine lung aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography.
Unit Definition:One trypsin inhibitor unit (EPU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin Unit = 1800 KIU.
MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | Clear, colorless to yellowish liquid |
| Protein Concentrate | ≥ 5.0 |
| Specific Activity | ≥ 3.0EPU /mg protein |
| Absorbance (277nm) | ≤ 0.8(3 EPU/ml in water) |
| Purity (HPLC) | ≥95% |
UNIT DEFINITION:One trypsin inhibitor unit (EPU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.
A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin Unit
RECOMMEND USAGE
Effective concentration is equimolar with protease. The recommend binding is pH＞6.0 and it will dissociate at pH <3.0.
Storage Instructions
Recombinant aprotinin should be stored under -20°C or below in sealed container.It is stable within 24 months.
Contact Person: Miss Eland
Tel: +8613482039151
