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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RSPA05 |
| Minimum Order Quantity: | 10mg |
|---|---|
| Price: | 79$/10mg |
| Packaging Details: | ice packaging |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Source: | E.Coli | Purified: | HPLC |
|---|---|---|---|
| Bioburden: | No Organisms Detected. | 12% SDS-PAGE: | Single Major Band |
| Related Product: | Recombinant Porcine Trypsin | Purity: | 95% |
| SDS-PAGE (MW): | 25000±1000 Daltons | ||
| High Light: | SPA protein A,protein A column |
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Recombinant Protein A Purified By Non-Antibody Column
Recombinant Protein A
Application
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.
Product Specification
|
Test/Method |
Specification |
|
Bioburden |
No organisms detected. |
|
12% SDS-PAGE |
Single major band |
|
Coomassie Stain |
25kD |
|
UV Spectrum |
E275/250 ≥1.2, E0.1% at 275nm=0.17-0.23 |
|
UV spectrometry |
No DNA or RNA detected |
|
Triton Content |
No Triton contaminant. |
|
Format |
Lyophilized |
|
pH stable range |
pH 1-13 |
PRODUCT ADVANTAGE
1) Non-antibody column purification, the product without any other antibodies such as IgG and other pollution.
2) SPA-25 is our own research and development products, pH tolerance range, can withstand 0.5 M NaOH and 0.5M HCl treatment, no degradation, antibody binding capacity unchanged.
3) The product has a specially designed link for the purification of the column, can be directly used for the preparation of protein A column.
4) Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
5) High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
6) Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
7) Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
8) Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
Storage Instructions
Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.
Usage
For Research or Manufacturing Purpose Only. Not for Human.
Contact Person: Miss Eland
Tel: +8613482039151
