Place of Origin: | China, Shanghai |
Brand Name: | YAXINBIO |
Certification: | NQA ISO 9001:2015 |
Model Number: | RSPA05 |
Minimum Order Quantity: | 10mg |
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Price: | 79$/10mg |
Packaging Details: | 10mg, 100mg, 1g; ice packaging, carton |
Payment Terms: | T/T |
Supply Ability: | 1000g per week |
Source: | E.Coli | Purified: | HPLC |
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Bioburden: | No Organisms Detected | 12% SDS-PAGE: | Single Major Band |
Coomassie Stain: | 25kD | UV Spectrum: | E275/250≥1.2, E0.1% At 275nm=0.14-0.20 |
High Light: | SPA protein A,protein A column |
SPA Binding to IgG Structure of Different Antibodies Protein A and PCR amplification results
Recombinant Protein A
Application
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.
Product Specification
Test/Method |
Specification |
Coomassie Stain |
25kD |
UV Spectrum |
E275/250 ≥1.2, E0.1% at 275nm=0.17-0.23 |
UV spectrometry |
No DNA or RNA detected |
Triton Content |
No Triton contaminant |
Format |
Lyophilized |
pH stable range |
pH 1-13 |
STABILITY OF STORAGE AND TRANSPORT
APPLICATION
The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.
Contact Person: Miss Eland
Tel: +8613482039151