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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | REK08 |
| Minimum Order Quantity: | 100U |
|---|---|
| Price: | 65$/100u |
| Packaging Details: | ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000ku per week |
| Product: | Recombinant Enterokinase | Manufacturer: | YaxinBio |
|---|---|---|---|
| Specific Activity: | One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein. | Fusion Protein Concentration: | 0.1-1mg/ml (total Protein Content: 0.5-1.0mg) |
| Cutting Condition: | Given An Example: 25mM Tris-HCl 8.0 | RELATED PRODUCT: | Recombinant Carboxypeptidase B |
| High Light: | bovine enterokinase,enterokinase enzyme |
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Recombinant Enterokinase, Expressed in E.coli, Recombinant Enterokinase, Animal Origin Free, Supplier
Recombinant Enterokinase
Description
Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.
Common Components Influence The Action of Enterokinase
＞200mM imidazole or ＞200mM NaCl or ＞5%glycerin, the reaction may be effected.The following suggestions are given:
1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.
2) If the dialysis is inconvenient, please dilute the sample to ＜100mM imidazole, ＜50mMNaCl, ＜5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).
3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.
Main Features
|
Source |
E.Coli |
|
M.W. |
25,850 Da |
|
Specific Activity |
One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to 16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein. |
|
Storage Temperature |
-20°C or below. |
|
Stability |
Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing. |
Product Information
|
Product |
Activity |
Packaging |
Manufacturer |
|
Recombinant Enterokinase |
≥5 u/μl |
100u;500u;1ku; 10ku |
YaxinBio |
Contact Person: Miss Eland
Tel: +8613482039151
