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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | REK08 |
| Minimum Order Quantity: | 100U |
|---|---|
| Price: | 65$/100U |
| Packaging Details: | ice packaging |
| Payment Terms: | T/T |
| Supply Ability: | 1000ku per week |
| Source: | E. Coli | Time: | Overnight Or 16h-24h For Digestion. |
|---|---|---|---|
| Cat.No: | REK08 | Specific Activity: | One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein. |
| Fusion Protein Concentration: | 0.1-1mg/ml (total Protein Content: 0.5-1.0mg) | Cutting Condition: | Given An Example: 25mM Tris-HCl 8.0 |
| High Light: | bovine enterokinase,enterokinase enzyme |
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Recombinant Bovine Enterokinase Cleaving DDDDK
Recombinant Bovine Enterokinase
CAS:9017-74-8
EC:3.4.21.9
Source: bovine enterokinase, expressed in E. Coli
Description
YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.
MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | Clear,colorless to yellowish liquid |
| Activity | ≥5 u/μl |
| Purity(SDS-PAGE) | Single major band |
| Molecular Weight(SDS-PAGE) | 25.8 ±2.6 kDa |
UNIT DEFINITION:One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to 16 hours to get 95% completion at 25°C in 25 mMTris-HCl, pH 8.0. Substrate: a special fusion protein.
Advantages
| Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK) |
| No any other contaminated proteases, no non-specific cutting sites |
Product Information
|
Product |
Activity |
Packaging |
Manufacturer |
|
Recombinant Enterokinase |
≥5 u/μl |
100u;500u;1ku; 10ku |
YaxinBio |
Contact Person: Miss Eland
Tel: +8613482039151
