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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RHT03 |
| Minimum Order Quantity: | 10mg |
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| Price: | 89$/10mg |
| Packaging Details: | 1mg, 10mg, 100mg, 1g; ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Source: | E. Coli | Cat. No.: | RHT03 |
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| Manufacturer: | YaxinBio | Contaminant Activity: | No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant. |
| Related Product: | Recombinant Porcine Trypsin | Packaging: | 10mg,100mg Or Bulk |
| High Light: | trypsin digestion,stem cell culture |
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Animal Origin Free, Recombinant Human Trypsin, for Treating Cell
Recombinant Human Trypsin
CAS: 9002-07-7
EC: 3.4.21.4
Source: human trypsin, expressed in E. Coli.
Description
1.Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues.pH7.0-9.0 is the optimum pH. Serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA can inhibite the trypsin enzyme.
2.Recombinant human trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | White or off white powder |
| Specific activity | ≥2500 USP units/mg pro. |
| Protein Content | ≥ 90% |
| Purity(SDS-PAGE) | Single major band |
| Molecular Weight(SDS-PAGE) | 24.0±2.4 kDa |
| Purity(HPLC) | ≥ 95% |
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
RECOMMEND USAGE
Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
Storage Instructions
Recombinant human trypsin lyophilized powder should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland
Tel: +8613482039151
