Trypsin, Stem Cell Culture, Clinic Cell Therapy, Animal Origin Free, Human Trypsin

Trypsin, Stem Cell Culture, Clinic Cell Therapy, Animal Origin Free, Human Trypsin

Human Tryspsin

Description

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.

Recombinant Human Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

Main Features

Unit Definition

One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH 7.6 and 25℃, with BAEE as a substrate (1cm light path).

Storage Instructions

Recombinant human trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.