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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RHT03 |
| Minimum Order Quantity: | 10mg |
|---|---|
| Price: | 89$/10mg |
| Packaging Details: | ice packaging, carton |
| Delivery Time: | 7 days |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Recombinant Human Trypsin 2 | Source: | E. Coli |
|---|---|---|---|
| Purified By: | HPLC | Contaminant Activity: | No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant. |
| Related Product: | Recombinant Porcine Trypsin | Purity: | > 99% |
| Impact: | Dissociation Cells | ||
| High Light: | dissociated cell culture,lyophilized powder |
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Cell Dissociation Solution, Recombinant Human Trypsin for Enzymatic Hydrolysis of Protein
Cell Dissociation Solution - Recombinant Human Trypsin
Description
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.
Recombinant Human Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
Advantages
Animal origin free:The use of recombinant Human Trypsin eliminates the risk of virus presence, and of any other potential adventitious agents found in animal pancreas-derived trypsin. YaxinBio Recombinant Human Trypsin belongs to the AOF level 3.
Recombinant human trypsin:The amino acid sequence is the same as the Human Trypsin 2.
Stable:A sterile recombinant human trypsin lyophilized eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant human trypsin provides increased specificity and eliminates
contaminating activities found in lower purity enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
3) Purity is more than 95% by HPLC.
Main Features
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Format |
White or White-like lyophilized |
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Specific activity |
≥ 2500 USP u/mg pro. |
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Purity |
≥ 95% by HPLC |
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Contaminant activity |
No chymotrypsin, carboxypeptidase A, and other protease contaminant. |
Unit Definition :One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Storage Instructions
Recombinant human trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland
Tel: +8613482039151
