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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RHT03 |
| Minimum Order Quantity: | 10mg |
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| Price: | 89$/10mg |
| Packaging Details: | 1mg, 10mg, 100mg, 1g; ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Recombinant Human Trypsin 2 | Packaging: | 10mg,100mg Or Bulk |
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| Specific Activity: | ≥2500 USP U/mg Pro. | Contaminant Activity: | No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant. |
| Related Product: | Recombinant Porcine Trypsin | Manufacturer: | YaxinBio |
| High Light: | trypsin digestion,stem cell culture |
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Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
Recombinant Human Trypsin
Description
| 1.Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA. |
| 2.Recombinant human trypsin is protein expressed in E.coli through a genetically engineered and purified by high pressure liquid chromatography. Enzyme activities such as carboxypeptidase A and chymotrypsin are not contained in the trypsin. No protease inhibitors such as PMSF are contained in the preparation. |
MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | White or off white powder |
| Specific activity | ≥2500 USP units/mg pro. |
| Protein Content | ≥ 90% |
| Purity(HPLC) | ≥ 95% |
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
RECOMMEND USAGE
Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
Advantages
Animal origin free:The use of recombinant human trypsin eliminates the risk of virus presence and any other potential adventitious agents found in animal pancreas-derived trypsin. YaxinBio recombinant human trypsin belongs to the AOF level 3.
Recombinant human trypsin:The amino acid sequence is the same as the human trypsin 2.
Stable:A sterile recombinant human trypsin lyophilized eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
| Recombinant human trypsin provides increased specificity and eliminates
contaminating activities found in lower purity enzymes. |
| No other contaminating proteases such as chymotrypsin or carboxypeptidase A. |
| Purity is more than 95% by HPLC. |
Contact Person: Miss Eland
Tel: +8613482039151
