Human Trypsin Solution Pre-formulated Concentration to Dissociate Cells

Human Trypsin Solution Pre-formulated Concentration to Dissociate Cells

Description

Special, animal components-free (ACF) recombinant trypsin solutions, developed as an alternative to porcine trypsin. The solutions do not contain any chymotrypsin, carboxypeptidase A, and other protease contaminant. Recombinant Trypsin Solution formulations were developed for efficient dissociation of adherent cell types from surfaces and tissues and were optimized for sensitive cells, such as primary human mesenchymal stem cells (hMSC). The addition of EDTA usually accelerates the dissociation phase. Recombinant Trypsin Solutions are pure enzyme solutions, which help maximize the yield of functionally viable cells from culture vessels, while preventing the toxicity effect induced by other non desirable proteases. In addition, recombinant trypsin eliminates the risk of viruses, or other potential adventitious agents found in animal derived components.

Advantages

Animal origin free:The use of recombinant Human Trypsin eliminates the risk of virus presence, and

of any other potential adventitious agents found in animal pancreas-derived trypsin. YaxinBio Recombinant Human Trypsin belongs to the AOF level 3.

Recombinant human trypsin:The amino acid sequence is the same as the Human Trypsin 2.

Stable:A sterile recombinant human trypsin lyophilized eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant human trypsin provides increased specificity and eliminates

contaminating activities found in lower purity enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

3) Purity is more than 95% by HPLC.

Main Features

Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a

reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

Storage Instructions

Recombinant human trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is

stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months

and above 90% activity remained after 10 times repeated freezing and thawing.