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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RPT0201 |
| Minimum Order Quantity: | 10mg |
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| Price: | 65$/10mg |
| Packaging Details: | ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Recombinant Porcine Trypsin | Physical Form: | White Lyophilized Powder |
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| Specific Activity: | ≥3800 USP U/mg Pro | Purity: | > 99% |
| Related Product: | Recombinant Protein A | ||
| High Light: | porcine trypsin,cell culture trypsin |
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Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry
Recombinant Trypsin
Description
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end
of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents, e.g.EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified
by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
Advantages
Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence,
and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant
Porcine Trypsin belongs to the AOF level 3.
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | White or off white, or yellowish powder |
| Specific activity | ≥3800 USP units/mg pro. |
| Purity(SDS-PAGE) | Single major band |
| Molecular Weight(SDS-PAGE) | 24.0±2.4 kDa |
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Recommend Usage
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
Storage Instructions
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable
within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and
above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland
Tel: +8613482039151
