|Place of Origin:||China, Shanghai|
|Certification:||NQA ISO 9001:2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||ice packaging, carton|
|Supply Ability:||1000g per week|
|Product:||Recombinant Porcine Trypsin||Cat. No.:||RPT0201|
|Specific Activity:||≥3800 USP U/mg Pro||Purity:||> 99%|
|Related Product:||Recombinant Lipase A|
cell culture trypsin
Serine Protease, Lysine Protease, Arginine Protease, Recombinant Trypsin
Trypsin can autocatalytically activate more trypsinogen to trypsin. Trypsin consists of a single chain polypeptide of 223 amino acid residues. This native form of trypsin is refered to as β-trypsin. Autolysis
of β-trypsin (which is cleaved at Lys- Ser in the bovine sequence) results in α-trypsin which is held together by disulfide bridges. Trypsin is a member of the serine protease S1 family. The active site amino acid residues of trypsin include His and Ser Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents,e.g.EDTA.
(1)Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
(2)Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
(3)High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
(4)Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
(5)Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
(6) Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
NLT 70% β-trysin, NMT 20% α-trypsin
No chymotrypsin, carboxypeptidase A, and other protease contaminant.
One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant human trypsin
Contact Person: Miss Eland