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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RPT0201 |
| Minimum Order Quantity: | 10mg |
|---|---|
| Price: | 65$/10mg |
| Packaging Details: | 100ml, 500ml; ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Manufacturer: | YaxinBio | Cat. No.: | RPT0201 |
|---|---|---|---|
| Contaminant Activity: | No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant. | Physical Form: | White Lyophilized Powder |
| Related Product: | Recombinant Human Trypsin | Impact: | Dissociate Cells |
| Source: | Expressed In E.coli. | ||
| High Light: | dissociated cell culture,trypsin cell culture |
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Cell Dissociation Solution, Expressed in E.coli, Dissociate Cells
Recombinant Trypsin Solution
Description
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
Advantages
Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Main Features
|
Source |
E. Coli |
|
Purified by |
HPLC |
|
Specific activity |
≥3800 USP units/mg pro |
|
Purity(RP-HPLC) |
NLT 70% β-trysin, NMT 20% α-trypsin |
|
Contaminant activity |
No chymotrypsin, carboxypeptidase A, and other protease contaminant. |
Unit Definition :One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Recommend Usage
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.
Storage Instructions
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland
Tel: +8613482039151
