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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RPT0201 |
| Minimum Order Quantity: | 10mg |
|---|---|
| Price: | 65$/10mg |
| Packaging Details: | ice packaging, carton |
| Delivery Time: | 6 days |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Recombinant Porcine Trypsin | Cat. No.: | RPT0201 |
|---|---|---|---|
| Physical Form: | White Or White-like Lyophilized Powder | Additives: | Carbohydrates |
| Protein Content: | 35% ～ 70% | Related Product: | Recombinant Trypsin Solution |
| High Light: | porcine trypsin,trypsin enzyme |
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Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
Recombinant Trypsin
Description
Trypsin
Enzyme Commission (EC) Number: 3.4.21.4
Molecular Weight: 23.3 kDa (porcine)
Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
pI: 10.1 - 10.5(porcine)
Trypsinogen
Molecular Weight: 24 kDa (porcine)
Extinction Coefficient: E1% = 14.4 (280 nm)
pI: 9.3 (porcine)
Procedure
Pipette (in milliliters) the following reagents into suitable quartz cuvettes:
| Test | Blank | |
| Reagent B (BAEE) | 3.00 | 3.00 |
Equilibrate to 25 °C. Monitor the A253nm until constant, using a suitably thermostatted spectrophotometer. Then add:
| Test | Blank | |
| Reagent C (HCl) | ---- | 0.20 |
| Reagent D (Enzyme Solution) | 0.20 | ---- |
Immediately mix by inversion and record the increase in A253nm for approximately 5 minutes. Obtain the ΔA253nm/minute using the maximum linear rate for both the Test and Blank.
Calculation
| BAEE Units/ml enzyme = | (ΔA253nm/min Test - ΔA253nm/min Blank)(df) |
| (0.001) (0.20) |
df = Dilution factor
0.001 = The change in A253nm/minute per unit of Trypsin at pH 7.6 at 25 °C in a 3.2 ml reaction mix
0.20 = Volume (in milliliters) of enzyme used
| Units/mg solid= | units/ml enzyme |
| mg solid/ml enzyme |
Advantages
Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin.
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Application
Use Trypsin recombinant, Proteomics Grade, to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.
Recommend Usage
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.
Contact Person: Miss Eland
Tel: +8613482039151
