Place of Origin: | China, Shanghai |
Brand Name: | YAXINBIO |
Certification: | NQA ISO 9001:2015 |
Model Number: | V813 |
Minimum Order Quantity: | 50ug |
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Price: | 65$/50ug |
Packaging Details: | ice packaging |
Payment Terms: | T/T |
Supply Ability: | 1000g per week |
Name: | Endoproteinase Glu-C | Species: | Staphylococcal Serine Proteinase |
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CAS: | 9004-07-3 | Band: | YAXINBIO |
Specific Activity: | ≥ 5.0 U /mg Pro. | Purity(SDS-PAGE): | Single Major Band |
High Light: | human chymotrypsin,enzyme chymotrypsin |
CAS 66676-43-5, Staphylococcus Aureus v8 Protease, Endoproteinase Glu C, Staphylococcal Serine Proteinase
Sequencing Grade V8 Protease
Catalog Number:V813
CAS:66676-43-5
EC:3.4.21.19
Full Name:Sequencing grade recombinant V8 protease;Staphylococcus aureus strain V8
Synonyms:V8 protease;Endoproteinase Glu-C;Endoproteinase Asp-C
Storage temperature: 2-8°C
Source: Recombinant V8 protease is a genetically engineered protein expressed in E.coli
DESCRIPTION
Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues.The optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors are Phosphoric acid diisopropyl ester fluoride (DFP) and alpha 2 - macroglobulin and Na-p-tosyl-L-lysine chloromethyl ketone (TLCK).
SPECIFIC ACTIVITY
Approx.5U/mg at 25℃ with Z-Phe-Leu-Glu-4-nitranilide as the substrate(approx.500U/mg at 37℃ with casein as the substrate).
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant V8 protease should be stored under 2-8°C in sealed container.It is stable within 6 months after dissolved with1mM HCl and 50mM HAC at -20°C or below. It is stable within 2 months after dissolved with 1mM HCl and 50mM HAC at 4°C.85% activity above can be kept after dissolved with 1mM HCl and 50mM HAC at 25°C or 37°C 12h. It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
Contact Person: Miss Eland
Tel: +8613482039151