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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RCPB01 |
| Minimum Order Quantity: | 10mg |
|---|---|
| Price: | 150$/10mg |
| Packaging Details: | 1mg, 10mg, 100mg, 1g; ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Recombinant Carboxypeptidase B | Brand: | YaxinBio |
|---|---|---|---|
| Protein Content: | 35% ～ 50% | Specific Activity: | ≥120 Units/mg Pro.; ≥170 Units/mg Pro |
| Packaging: | 10mg,100mg,1g,bulk | Contaminant activity: | No Chymotrypsin,carboxypeptidase A,and Other Protease Contaminant.Less Than 10ppm Of Recombinant Trypsin. |
| High Light: | trypsin endopeptidase,carboxypeptidase enzyme |
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Enzymes, Recombinant Carboxypeptidase B, Expressed in E.coli, Digestion of Protein and Polypeptide
Recombinant Carboxypeptidase B
Description
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
Main Features
|
Source |
E. Coli |
|
Purified by |
HPLC |
|
Format |
White or white-like or yellowish lyophilized |
|
Additives |
Tris salts, NaCl salts and carbohydrates |
|
Purity |
Single main band on SDS-PAGE |
|
M.W. |
33.8kD |
|
Contaminant activity |
Less than 10 ppm of recombinant trypsin. |
Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.
Advantages
① Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.
② Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
③ High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host protein residues is less than the limits of biological products.
④ Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.
⑤ Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
⑥ Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
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Contact Person: Miss Eland
Tel: +8613482039151
