Place of Origin: | China, Shanghai |
Brand Name: | YAXINBIO |
Certification: | NQA ISO 9001:2015 |
Model Number: | RCPB01 |
Minimum Order Quantity: | 10mg |
---|---|
Price: | 150$/10mg |
Packaging Details: | ice packaging, carton |
Delivery Time: | 7 work days |
Payment Terms: | T/T |
Supply Ability: | 100000g per week |
Source : | E. Coli | Purified by: | HPLC |
---|---|---|---|
Physical form : | White Or White-like Lyophilized Powder | Additives : | Tris Salts, NaCl Salts And Carbohydrates |
Protein content : | 35% ~ 50% | Purity : | > 95% |
Nature: | Excision Enzymes | Advantage: | Animal Oriigin Free |
High Light: | trypsin endopeptidase,carboxypeptidase enzyme |
Recombinant Carboxypeptidase B, For Excision of Peptide, Animal Origin Free
Recombinant Carboxypeptidase B
Description
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
Applications
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
PRODUCT USAGE
The production of recombinant insulin and its analogues;
the quality test of recombinant antibodies;
the determination of C-terminal amino acids of proteins;
the production of other recombinant polypeptides;
and the enzymatic synthesis of certain specific compounds.
Contact Person: Miss Eland
Tel: +8613482039151