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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RCPB01 |
| Minimum Order Quantity: | 10mg |
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| Price: | 150$/10mg |
| Packaging Details: | ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Recombinant Carboxypeptidase B | Brand: | YaxinBio |
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| Protein Content: | 35% ～ 50% | Specific Activity: | ≥170 Units/mg Pro |
| Purity: | > 95% | Cat. No.: | RCPB01 |
| High Light: | sequencing grade modified trypsin,carboxypeptidase enzyme |
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Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade
Recombinant Carboxypeptidase B
CAS: 9025-24-5
EC: 3.4.17.2
Source: Expressed in E. Coli.
Description
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
Main Features
| Source | E. Coli |
| Purified by | HPLC |
| Format | White or white-like or yellowish lyophilized |
| Additives | Tris salts, NaCl salts and carbohydrates |
| Purity | Single main band on SDS-PAGE |
| M.W. | 33.8kD |
| Contaminant activity | Less than 10 ppm of recombinant trypsin. |
Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.
Advantages
Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or of any other potential adventitious agents found in animal-derived carboxypeptitase B
Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.
High purity:
1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.
2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.
3)Less than 10ppm of recombinant trypsin.
Recommend Usage
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Storage Instructions
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland
Tel: +8613482039151
