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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RCPB01 |
| Minimum Order Quantity: | 10mg |
|---|---|
| Price: | 150$/10mg |
| Packaging Details: | ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Recombinant Carboxypeptidase B | Cat. No.: | RCPB01 |
|---|---|---|---|
| Specific activity: | ≥170 U/mg Pro | Additives: | Tris Salts, NaCl Salts And Carbohydrates |
| Protein content: | 35% ～ 60% | Purity: | > 95% |
| High Light: | trypsin endopeptidase,carboxypeptidase enzyme |
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Carboxypeptidase B, For Cell Culture and Protein Digestion
Recombinant Carboxypeptidase B
DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.
Main Features
|
Source |
E. Coli |
|
Purified by |
HPLC |
|
Specific activity |
≥170 units/mg pro. |
|
Purity |
Single main band on SDS-PAGE |
|
M.W. |
33.8kD |
|
Contaminant activity |
Less than 10 ppm of recombinant trypsin. |
Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.
Recommend Usage
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
Storage Instructions
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland
Tel: +8613482039151
