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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RCPB01 |
| Minimum Order Quantity: | 10mg |
|---|---|
| Price: | 150$/10mg |
| Packaging Details: | ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Purified By: | HPLC | Physical Form: | White Or White-like Lyophilized Powder |
|---|---|---|---|
| Additives: | Tris Salts, NaCl Salts And Carbohydrates | Cat. No.: | RCPB01 |
| Specific Activity: | ≥170 U/mg Pro | Purity: | ≥95% |
| Impact: | Mass Spectrometry | ||
| High Light: | sequencing grade modified trypsin,trypsin endopeptidase |
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Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro.
Recombinant Carboxypeptidase B
Description
Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 33.8 kda, optimun pH-7.9,
and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited
by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA,1,10-phenanthroline.
Synonyms: Carboxypeptidase B, Cpb1, Cpb.
Advantages
Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or any other potential adventitious agents found in animal-derived carboxypeptitase B
Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.
High purity:
1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.
2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.
3)Less than 10ppm of recombinant trypsin.
Main Features
|
Source |
E. Coli |
|
Protein content |
35% ～ 60% |
|
Purity |
Single main band on SDS-PAGE |
|
M.W. |
33.8kD |
|
Contaminant activity |
Less than 10 ppm of recombinant trypsin. |
Unit Definition
One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.
Recommend Usage
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Storage Instructions
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland
Tel: +8613482039151
