|Place of Origin:||China, Shanghai|
|Certification:||NQA ISO 9001:2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||ice packaging, carton|
|Supply Ability:||1000g per week|
|Product:||Recombinant Carboxypeptidase B||Cat. No.:||RCPB01|
|Specific Activity:||≥170 U/mg Pro||Packaging:||10mg,100mg,1g,bulk|
Carboxypeptidase B, Producing Recombinant Polypeptide C-terminal lysine EC 22.214.171.124
Recombinant Carboxypeptidase B
Source: Expressed in E. Coli.
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
|Source||Recombinant E. coli|
|Appearance||White or off white, or yellowish powder|
|Specific activity||≥170 units/mg pro.|
|Purity(SDS-PAGE)||Single major band|
|Molecular Weight(SDS-PAGE)||33.8±3.4 kDa|
UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.
Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or of any other potential adventitious agents found in animal-derived carboxypeptitase B
Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.
1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.
2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.
3)Less than 10ppm of recombinant trypsin.
Contact Person: Miss Eland