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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RSPA05 |
| Minimum Order Quantity: | 10mg |
|---|---|
| Price: | 79$/10mg |
| Packaging Details: | 10mg, 100mg, 1g; ice packaging, carton |
| Delivery Time: | 5-8 weekdays |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Substrate: | Porous Polystyrene-divinylbenzene | Particle Size: | 40 Um |
|---|---|---|---|
| Ligand: | Recombinant Protein A | Recommend Flow Rate: | 300-700 Cm/h |
| DBC: | At Least 300 Mg IgG/mL Media At 500 Cm/h | Maximum Pressure: | 1500 Psi |
| Brand: | YaxinBio | ||
| High Light: | protein A column,purified protein |
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Enzyme Markers Monoclonal and Polyclonal Antibody Purification
Recombinant Protein A
Catalog Number:RSPA05
CAS:91932-65-9
Storage temperature: 2-8℃
Source:Protein A mutant of Staphylococcus aureus , expressed in E. coli
1.DESCRIPTION
Protein A is a membrane protein of Staphylococcus aureus and can specifically bind to antibodies. Protein A affinity chromatography columns have become widely used as affinity columns for purified antibodies from ascites, serum and cell culture isolated or purified extracts of antibodies from different subtypes of mammals or genetically engineered recombinant proteins containing antibody Fc fragments. alkali protein A is our company independent research and development production, with independent intellectual property rights. Recombinant protein A is expressed by E. coli and purified by chromatography to give a purity of 95% or more. The purification process does not use antibody column affinity chromatography to avoid the possibility of incorporation of unrelated IgG in the product.
2.MAIN FEATURES
| Appearance | White or off white powder |
| Solubility | Clear liquid |
| Bioburden | ≤ 1 cfu/ mg |
| SDS-PAGE | Single major band |
| SDS-PAGE (MW) | 25000±1000 Daltons |
| UV Spectrum | E275/250 ≥ 1.2 |
| E0.1% at 275nm = 0.17-0.23 | |
| Purity (HPLC) | ≥ 95% |
3.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.
After dissolved with pure water, it should be stored under -20℃.
Stability of transport: The product is stable by blue ice insulation transport.
4.APPLICATION:
The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.
5.Product Specification
|
Test/Method |
Specification |
|
Bioburden |
No organisms detected. |
|
12% SDS-PAGE |
Single major band |
|
Coomassie Stain |
25kD |
|
UV Spectrum |
E275/250 ≥1.2, E0.1% at 275nm=0.17-0.23 |
|
UV spectrometry |
No DNA or RNA detected |
|
Triton Content |
No Triton contaminant. |
|
Format |
Lyophilized |
|
pH stable range |
pH 1-1 |
6.Storage Instructions
Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.
7.Usage
For Research or Manufacturing Purpose Only. Not for Human.
8.RELATED PRODUCTS
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant human trypsin
Recombinant enterokinase
Contact Person: Miss Eland
Tel: +8613482039151
