Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

Descirption

Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

CAS: 9004-07-3
EC: 3.4.21.1

Source: human chymotrypsin, expressed in E. Coli.

Main Features

Application

1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.

Recommend Usage

For peptide digestion, use a ratio (w/w) of approximately 1:20-1:100 for chymotrypsin:peptide. Perform peptide digests in 40 mM Tris-HCl pH7.8 containing 5 mM CaCl₂, at 30 °C. Self digestion may occur if temperatures above 37°C.

Storage Instructions

Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.