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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | REK08 |
| Minimum Order Quantity: | 100U |
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| Price: | 65$/100u |
| Packaging Details: | 100U, 500U, 1000U, 10KU, 100KU, 1MU; ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000ku per week |
| Source: | E. Coli | M.W.: | Theoretical MW: 25,850 Da;The Apparent MW On SDS-PAGE: About 27,000 Da |
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| Storage: | Store At -20°C After Delivery. | Specific Activity: | One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein. |
| Stability: | Keep Cool With Blue Ice During Shipping.Remained Stable At 25°C For One Week Without Activity Lost. Can Be Stable After Several Times Of Freeze-thaw. | Cat.No: | REK08 |
| High Light: | bovine enterokinase,recombinant enzymes |
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Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery
Recombinant Enterokinase
Catalog Number:REK08
CAS:9017-74-8
EC:3.4.21.9
Storage temperature: -20°C or below
Source: Recombinant enterokinase is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant enterokinase is identical to bovine enterokinase light chain
1.Description
Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.
2.MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | Clear,colorless to yellowish liquid |
| Activity | ≥5 u/μl |
| Purity(SDS-PAGE) | Single major band |
| Molecular Weight(SDS-PAGE) | 25.8 ±2.6 kDa |
UNIT DEFINITION:One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.
3.Advantages
4. STABILITY OF STORAGE AND TRANSPORT
(1)Stability of storage: Recombinant enterokinase should be stored under -20°C in sealed container.It is stable within 24 months.If stored at 25°C ,it is stable within one week.It is no activity loss after 5 times repeated freezing and thawing.
(2)Stability of transport: The product is stable by blue ice insulation transport.
Contact Person: Miss Eland
Tel: +8613482039151
