High Purity Recombinant Soybean Trypsin Inhibitor For Cell Culture 90%

High Purity Recombinant Soybean Trypsin Inhibitor For Cell Culture ≥90%

1.Description

Soybean trypsin inhibitor is a serine protease inhibitor, which can inhibit the activity of trypsin, chymotrypsin, and plasmin. The ability to inhibit trypsin is higher than that of chymotrypsin and plasmin. A suitable pH range for inhibiting trypsin is pH 6.0-10.0. Inhibitors and protease can be effectively combined in equimolar amounts to form stable complexes to block the active site of the enzyme. Recombinant soybean trypsin inhibitor was expressed by recombinant E.coli and contains no animal-derived contamination and no other protease.

2.Product Features

(1) Appearance: This product is white, off-white, off-yellow powder with a loading of 1.0 and 10.0 mg / branch (based on enzyme protein content).

(2) Specific enzyme activity: ≥1.0 U/mg pro.

(3) HPLC purity identification: the main peak purity by area normalization method is not less than 90%.

Inhibitor enzyme activity unit: The ability to inhibit the activity of a trypsin unit is called an inhibitor activity unit (U).

Trypsin unit: One trypsin unit (micro katal) is defined to hydrolyze 1 μmol of N-benzoyl-L-arginine ethyl ester (BAEE) per second.

3.Product Instruction

Inhibitors and protease can be combined in equimolar amounts, the recommended pH for combination is pH>6.0 , inhibitors and protease won’t be combined when pH<5.0.

4.Stability

Stability of storage: Lyophilized powder is stable for 24 months at 2-8℃

Stability of transportation: Blue ice insulation transportation, stable activity.

5.Product Advantage

Animal origin free: The product is produced by recombination and no any animal source material is used in the production process. No exogenous virus contamination, safe to use.

Stable quality: Stable and continuous batch supply, little difference between batches of products, stable quality. Production process follows NSF

Complete quality documents: According to customer requirements, relevant regulatory support documents can be provided.

6.Product Usage

Soybean trypsin inhibitor is a serine protease inhibitor. It can form a stable complex with protease to block the active site of the enzyme. Such as: inhibition of serine protease activity in recombinant protein production; cell culture and other biotechnology processes.