|Place of Origin:||China, Shanghai|
|Certification:||NQA ISO 9001:2015|
|Minimum Order Quantity:||Negotiation|
|EC::||18.104.22.168||Molecular Weight:||27.9±2.8 KDa|
|Endotoxin:||<0.25 EU/1000 U By LAL||Optimum Temperature:||37℃|
High Purity Super Nuclease For Genetic Engineering Expressed In E.Coli
Benzonase Nuclease, Synonyms:Benzonase Endonuclease,Super Nuclease,TurboNuclease,Universal Nuclease,a non-specific endonuclease derived from Serratia marcescens. Yaxin Benzonase Nuclease has a high activity and enzyme specificity, can cut between any nucleotides in the chain to completely digest the nucleic acid into 5'-monophosphate oligonucleotides with a length of 3-8 bases, which can degrade various forms of DNA and RNA (double-stranded, single-stranded, linear, circular, natural or denatured) under a wide range of conditions (6M Urea, 0.1M Guanidine HCl, 0.4%Triton X100, 0.1% SDS, 1mM EDTA, 1mM PMSF). This product has a wide range of uses, usually used in removing nucleic acids from biological products such as recombinant proteins, virus vaccines, and effectively reducing the viscosity of protein lysate samples such as cells, tissues, and microorganisms.
|Source||Recombinant E. Coli|
|Activity||≥ 250 U/μL|
|Buffer||20 mM Tris-HCL pH8.0, 2mM MgCl2, 2mM NaCl, 50% Glycerol|
|Endotoxin||<0.25 EU/1000 U by LAL|
One unit Nuclease is defined as the amount of enzyme that causes a ∆A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30min at 37°C, pH 8.0.
1. Cell processing: a. Remove the culture medium from adherent cells, wash with PBS, add 1-2uL totipotent nuclease in 1mL RIPA lysate (or other mammalian cell lysates), incubate at room temperature or on ice for 5-30min, collect the lysate After centrifugation, the supernatant can be used for downstream experiments. b. After collecting the suspended cells by centrifugation, add 1mL RIPA lysis solution (or other mammalian cell lysis solution) plus 1-2uL totipotent nuclease in the centrifuge tube, incubate at room temperature or on ice for 5-30min, collect the lysis solution, centrifuge and take it up The downstream experiment can be performed after clearing.
2. Tissue sample: After grinding 30-100mg of animal or plant tissue sufficiently, add 100-200uL of lysate, and add 0.5-1uL totipotent nuclease at the same time, incubate at room temperature or on ice for 5-30min, collect the lysate, centrifuge to take the supernatant You can perform downstream experiments.
3. Escherichia coli or other bacteria: After the bacteria are collected by centrifugation, they are lysed with lysate or grinded and broken, add 0.5-1uL of Almighty Nuclease per 1mL, incubate at room temperature for 5-30min, collect the lysate, centrifuge to take the supernatant and proceed downstream experiment.
Note: If the solution is a high-salt solution, acidic or alkaline, and contains a higher concentration of detergents and denaturants, the amount of enzyme or incubation time should be appropriately increased.
4.Stability of Storage
Storage stability: -20℃ within specification range for a period of at least 24 months .
Note: it is not recommended to store the product at -70℃ or below, since freezing the product will cause loss of acivity
This product is used to reduce the viscosity of cell, tissue or bacterial lysate; remove nucleic acid in recombinant protein/recombinant virus; remove nucleic acid interference when determining recombinant virus titer by real-time quantitative PCR; prevent cell clumping; reduce the viscosity of E. coli lysate to improve Difficulty in centrifugation and filtration in the purification process; increase the refolding rate of inclusion body proteins; preparation of two-dimensional gel electrophoresis samples.
Contact Person: Miss Eland