|Place of Origin:||China, Shanghai|
|Certification:||NQA ISO 9001:2015|
|Model Number:||trypsin solution|
|Minimum Order Quantity:||100ml|
|Packaging Details:||100ml; ice packing, carton|
|Name:||Cell Dissociation Solution||Advantage:||High Purity|
|Specific Activity:||More Than 3800USP Units /mg Pro||Band:||Yaxinbio|
trypsin cell culture
Cell Dissociation Solution, Specific Activity ≥3800 USP Units/mg Pro.
Recombinant Trypsin Solution
Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.
|Appearance||Clear, Colorless to Yellowish solution|
|Composition||10mM HCl,20mM CaCl2, pH2.0-3.0|
|Protein Concentration||70 ±10 mg/ml|
|Specific activity||≥ 3800 USP units/mg pro|
|Purity(RP-HPLC)||NLT 70% β-trysin, NMT 20% α-trypsin|
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
FEATURES AND BENEFITS
Recombinant trypsin,Animal component-free,eliminate the risk of virus and mycoplasma contamination
Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.
Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.
Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.
The trypsin solution, ready-to-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.
Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.
Good work without EDTA
No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.
Contact Person: Miss Eland