Recombinant Trypsin Solution, Optimized for Cell Dissociation

Recombinant Trypsin Solution, Optimized for Cell Dissociation

Recombinant Trypsin Solution

DESCRIPTION

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues.Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography.

FEATURES AND BENEFITS

Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination

Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.

High purity

Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.

High safety

The trypsin solution, ready-to-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.

Convenient

Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.

Good work without EDTA

No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.

METHOD OF USE

Step 1. Pour 1 ml buffer solution into EP tube containing trypsin powder.

Step 2. Dissolve the trypsin powder.

Step 3. Pour the solution into the vial containing buffer solution and mix. Best use it within 4 hours.

NOTES:

Note 1. Dissociate adherent cells under room temperature ; 37 ℃ is not recommended.

Note 2. If necessary, after dissolving, immediately dispense into suitable

containers, then stored under -20℃. To use it, dissolve it at room temperature.

Note 3. The content of trypsin is 2000 BAEE unit/ml as above prepared. If necessary, dilute it to 1000 unit/ml or others based on your experimental results.