High Activity Staphylococcus Aureus V8 Protease / Endoproteinase Asp-C

High Activity Staphylococcus Aureus V8 Protease / Endoproteinase Asp-C

Sequencing Grade V8 Protease

1. DESCRIPTION

Recombinant V8 protease is the serine protease family and can specifically hydrolyzing glutamic acid or aspartic acid residues carboxy side peptide bonds. The Glu carboxy terminal peptide bond can be identified and cleaved in CH₄COONH₄ buffer (pH 7.8) and NH₄HCO₃ (pH 4.0 ). The Glu or Asp carboxy terminal peptide bond can be Identified and cleaved in phosphate buffer (pH 7.8), and the hydrolysis rate of Glu was higher than the rate of Glu Asp. Activity of recombinant V8 protease is stable between pH 4.0-10.0, and the optimum pH is 8.0-8.5.

Recombinant V8 protease can be used alone or in combination with other proteases when used in protein digestion, peptide profiling and peptide mass fingerprinting analysis. The inhibitors of V8 protease are diisopropylfluorophosphate (DFP), α2-macroglobulin and Nα-P-tosyl-L-lysine chloromethyl ketone (TLCK).

2. MAIN FEATURES

UNIT DEFINITION: One activity unit is defined as the amount of enzyme required to catalytic substrate Z-Phe-Leu-Glu-4-nitranilide produced 1μmol of 4-nitroaniline per minute at 25°C, pH 7.8.

3. STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant V8 protease should be stored under at 2-8°C in sealed container. The product is stable within 6 months after dissolved with 1mM HCl and 50mM HAC and when stored under -20°C. It is stable within 2 months after dissolved with 1mM HCl and 50mM HAC when stored at 4°C. More than 85% activity can be kept after dissolved with 1mM HCl and 50mM HAC at 25°C or 37°C within 12h. There is no activity loss after 10 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.