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Product Details:
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| Product: | Sequencing Grade Trypsin | Cat. No.: | SRT0201 |
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| Physical Form: | Lyophilized Powder | Specific Activity: | >4500 USP Units/mg Pro |
| Purity: | One Single Main Band At 24kD By SDS-PAGE | Contaminant Activity: | No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant. |
| High Light: | sequencing grade modified trypsin,trypsin endopeptidase |
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Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein
sequencing grade trypsin
DESCRIPTION
YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
Main Features
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Source |
E. Coli |
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Format |
Liquid in 50mM HAc, or fluffy solid. |
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Protein concentration |
0.5mg/ml in 50mM HAc |
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Specific activity |
≥ 4500 USP units/mg pro |
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Purity(RP-HPLC) |
≥99% by HPLC |
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Contaminant activity |
Recombinant porcine trypsin. No any other proteases activities contaminant. |
Unit Definition
One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Application
Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
Recommend Usage
1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.
2. When using this product, thaw it at room temperature, and mix gently before use.
3. No activity lost when freeze-thaw 5 cycles.
Storage Instructions
1. The solution should be stored under -70℃, It is stable within 24 months.
2. Above 95% activity remained after 5 times repeated freezing and thawing.
3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.
Contact Person: Miss Eland
Tel: +8613482039151
