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Product Details:
Payment & Shipping Terms:
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| Product: | Recombinant Porcine Trypsin | Purified By: | HPLC |
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| Physical Form: | White Or White-like Lyophilized Powder | Additives: | Carbohydrates |
| Protein Content: | 35% ～ 70% | Related Product: | Chymotrypsin |
| High Light: | porcine trypsin,cell culture trypsin |
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Recombinant Trypsin Recombinant Porcine Trypsin 3800 USP u/mg pro Produce Insulin
Description
Specificity and Kinetics Trypsin will cleave peptides on the C-terminal side of lysine and arginine amino acid residues.
The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site.Trypsin will hydrolyze ester and amide linkages of several synthetic substrates.The pH optimum of trypsin is 7 - 10.Assay Method The activity of most Sigma preparations is determined by a continuous rate spectrophotometric assay and expressed in BAEE units.
Unit Definition: One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using
BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).
Conditions
Temp = 25 °C, pH = 7.6, A253nm, Light path = 1 cm
In a 3.2 ml reaction mix, the final concentrations are 63 mM sodium phosphate, 0.23 mM Nα-benzoyl-L-arginine ethyl ester, 0.06 mM hydrochloric acid, and 100 units trypsin.
Advantages
Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence,
and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant
Porcine Trypsin belongs to the AOF level 3.
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Main Features
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Source |
E. Coli |
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Specific activity |
≥3800 USP units/mg pro |
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Purity(RP-HPLC) |
NLT 70% β-trysin, NMT 20% α-trypsin |
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Contaminant activity |
No chymotrypsin, carboxypeptidase A, and other protease contaminant. |
Contact Person: Miss Eland
Tel: +8613482039151
