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Product Details:
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| Source: | E. Coli | Purified By: | HPLC |
|---|---|---|---|
| Physical Form: | White Or White-like Lyophilized Powder | Additives: | Carbohydrates |
| Protein Content: | 35% ～ 70% | Specific Activityactivity: | ≥3800 USP Units/mg Pro |
| Impact For: | Peptide Fingerprinting Analysis | Used For: | Protein Sequencing |
| High Light: | cell culture trypsin,trypsin enzyme |
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Recombinant Porcine Trypsin Peptide Fingerprinting Analysis Protein Sequencing
Description
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents, e.g.EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
CAS: 9002-07-7
EC: 3.4.21.4
Source: Porcine trypsin,expressed in E.coli.
Advantages
Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Main Features
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Source |
E. Coli |
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Purified by |
HPLC |
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Format |
White lyophilized |
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Specific activity |
≥3800 USP units/mg pro |
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Purity(RP-HPLC) |
NLT 70% β-trysin, NMT 20% α-trypsin |
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Contaminant activity |
No chymotrypsin, carboxypeptidase A, and other protease contaminant. |
Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Recommend Usage
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.
Storage Instructions
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Web:http://www.yaxinbio.com Email:Zhaozhi@yaxinbio.com
Contact Person: Miss Eland
Tel: +8613482039151
