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Recombinant Porcine Trypsin Aanimal origin free,Stable quality,Compliance with regulatory requirements

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Recombinant Porcine Trypsin Aanimal origin free,Stable quality,Compliance with regulatory requirements

Recombinant Porcine Trypsin Aanimal origin free,Stable quality,Compliance with regulatory requirements
Recombinant Porcine Trypsin Aanimal origin free,Stable quality,Compliance with regulatory requirements Recombinant Porcine Trypsin Aanimal origin free,Stable quality,Compliance with regulatory requirements Recombinant Porcine Trypsin Aanimal origin free,Stable quality,Compliance with regulatory requirements Recombinant Porcine Trypsin Aanimal origin free,Stable quality,Compliance with regulatory requirements Recombinant Porcine Trypsin Aanimal origin free,Stable quality,Compliance with regulatory requirements

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Product Details:
Place of Origin: China, Shanghai
Brand Name: YAXINBIO
Certification: NQA ISO 9001:2015
Model Number: RHT03
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 89$/10mg
Packaging Details: ice packaging
Payment Terms: T/T
Supply Ability: 1000 per week
Detailed Product Description
Source: E. Coli Purified By: HPLC
Physical Form: White Or White-like Lyophilized Powder Additives: Carbohydrates
Protein Content: 35% ~ 70% Specific Activityactivity: ≥3800 USP Units/mg Pro
Impact For: Peptide Fingerprinting Analysis Used For: Protein Sequencing
High Light:

cell culture trypsin

,

trypsin enzyme

Recombinant Porcine Trypsin Peptide Fingerprinting Analysis Protein Sequencing

 

 

Description

 

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents, e.g.EDTA.

Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

CAS: 9002-07-7
EC: 3.4.21.4

Source: Porcine trypsin,expressed in E.coli.

 

Advantages

 

Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.

Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

 

Main Features

 

Source

E. Coli

Purified by

HPLC

Format

White lyophilized

Specific activity

≥3800 USP units/mg pro

Purity(RP-HPLC)

NLT 70% β-trysin, NMT 20% α-trypsin

Contaminant activity

No chymotrypsin, carboxypeptidase A, and other protease contaminant.

Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

Recommend Usage

 

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.

 

Storage Instructions

 

Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

 

Recombinant Porcine Trypsin Aanimal origin free,Stable quality,Compliance with regulatory requirements 0

Web:http://www.yaxinbio.com Email:Zhaozhi@yaxinbio.com

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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