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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RPT0201 |
| Minimum Order Quantity: | 10mg |
|---|---|
| Price: | 65$/10mg |
| Packaging Details: | ice packaging, carton |
| Delivery Time: | 6 days |
| Payment Terms: | T/T |
| Supply Ability: | 1000000g per week |
| Product: | Recombinant Porcine Trypsin | Cat. No.: | RPT0201 |
|---|---|---|---|
| Contaminant Activity: | No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant. | Additives: | Additives |
| Related Product: | Recombinant Human Trypsin | Source: | Recombinant E.coli |
| High Light: | cell culture trypsin,trypsin enzyme |
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Recombinant Trypsin, Manufacturer, USP Standard Trypsin, Supplier
Recombinant Trypsin
Description
Enzyme Commission (EC) Number: 3.4.21.4
Molecular Weight: 23.3 kDa (bovine & porcine)
Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
pI: 10.1 - 10.5 (bovine)
Pipette (in milliliters) the following reagents into suitable quartz cuvettes:
| Test | Blank | |
| Reagent B (BAEE) | 3.00 | 3.00 |
Equilibrate to 25 °C. Monitor the A253nm until constant, using a suitably thermostatted
spectrophotometer.Then add:
| Test | Blank | |
| Reagent C (HCl) | — | 0.20 |
| Reagent D (Enzyme Solution) | 0.20 | — |
Immediately mix by inversion and record the increase in A253nm for approximately 5 minutes.
Obtain the ΔA253nm/minute using the maximum linear rate for both the Test and Blank.
Main Features
| Source | E. Coli |
| Purified by | HPLC |
| Physical form | White lyophilized powder |
| Specific activity | ≥3800 USP units/mg pro |
| Purity(RP-HPLC) | NLT 70% β-trysin, NMT 20% α-trypsin |
| Contaminant activity | No chymotrypsin, carboxypeptidase A, and other protease contaminant. |
Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute
in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Advantages
Stablility:
A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases
the chance of activity loss in the process of transport and storage.
High purity:
Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Contact Person: Miss Eland
Tel: +8613482039151
