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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | RTI16-S |
| Minimum Order Quantity: | 10mg |
|---|---|
| Price: | 180$/10mg |
| Packaging Details: | ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000g per week |
| Product: | Recombinant Aprotinin | Cat. No.: | 9087-70-1 |
|---|---|---|---|
| Manufacturer: | YaxinBio | Packaging: | 10mg,100mg, Or Bulk. |
| Purified By: | HPLC | Physical Form: | Liquid In 0.1 M NaCl Or White Lyophilized Powder |
| High Light: | serine protease inhibitors,aprotinin protease inhibitor |
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Recombinant Aprotinin, Expressed in E.coli, BPTI Inhibit Kallikrein, CAS 9087-70-1
Recombinant Aprotinin
DESCRIPTION
Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes and dissociating at pH <3.0. Effective concentration is equimolar with protease. YaxinBio recombinant aprotinin is a genetically engineered protein expressed in E.coli and purified by multi-column.The amino acid sequence of recombinant trypsin is identical to bovine lung derived aprotinin,with equivalent properties compared to native aprotinin and can replace native trypsin for using in a variety of biotechnological processes.
MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | Clear, colorless to yellowish liquid |
| Protein Concentrate | ≥ 5.0 |
| Specific Activity (EPU /mg protein) | ≥ 3.0 |
| Absorbance (277nm) | ≤ 0.8(3 EPU/ml in water) |
| Purity (HPLC) | ≥95% |
UNIT DEFINITION:One trypsin inhibitor unit (EPU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.
A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin Unit
Recombinant Animal Components Free
Recombinant aprotinin is expressed in E. Coli, and purified by HPLC. It contains no animal-derived components. This is a recombinant form of bovine lung aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography.
Main Features
|
Source |
E. Coli |
|
Format |
Liquid |
|
Specific activity |
≥ 3.0 EPU/mg pro. |
|
Purity |
≥ 95% |
|
Contaminant activity |
No any other protease contaminant. |
Unit Definition:One trypsin inhibitor unit (EPU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin Unit = 1800 KIU.
Contact Person: Miss Eland
Tel: +8613482039151
